What cassettes are included in a new instrument?
Multidrop Combi includes three types of dispensing cassettes, Standard tube cassette (Cat. No. 24072670), Small tube metal tip dispensing cassette (Cat. No. 24073295) and Small tube plastic tip dispensing cassette (Cat. No. 24073290).
What is the lifetime for a dispensing cassette?
The lifetime for the standard dispensing cassette is 3000-5000 plates of 96-well plates with a 100 µl volume, and for the small tube dispensing cassette 1000 plates of 384-well plates with a 5 µl volume.
It is hard to know when to change the dispensing cassette. Is there any help for this?
You can use Multidrop Combi SMART which utilises RFID technology in the instrument and dispensing cassettes. It counts the usage of the dispensing cassette and informs when the lifetime is fulfilled.
Can the dispensing cassette be autoclaved?
Yes, the standard tube cassette 50 times and the small tube cassette 10 times at 1 bar pressure at 121°C for 20 minutes.
What is the diameter of the cassette tip?
The inner diameter of the tip is 0.5 mm in the standard tube cassette, and 0.22 mm in the small tube cassette.
What is the diameter of cassette tubing?
The diameter of the standard tubing is 1.3 mm and diameter of the small tubing is 0.4 mm.
What are the smallest increment volumes with the small and standard tube cassettes?
The smallest increment is 0.5 µl for the small tube cassette and 5 µl for the standard tube cassette.
How often should the dispensing cassette be calibrated?
It is recommended to calibrate at least once a month depending on the frequency of the use and the liquids used. The cassette should also be calibrated if it is left under tension for prolonged periods.
How should the dispensing cassette be maintained?
The cleaning procedure always depends on the reagents dispensed. Always wash first with plenty of distilled water. Then you can clean with mild a detergent solution, for example, tween, triton or a cleaning solution (for example, 1% Micro-90).
Then wash again with plenty of distilled water. If you plan to run EtOH through the dispensing cassette, make sure there are no remnants of other liquids since EtOH will effectively precipitate proteins. Please see maintenance for further instructions.
Can I save a dispensing protocol?
Yes, the use of the Multidrop Combi allows you to save at least 100 dispensing protocols. First you need to proceed into the Save/Edit row (the last row in theMain menu), press OK and then the right pointing arrow.
Enter the name for protocol by selectiong the letters with the arrow keys and press OK to accept the name. The Protocol name appears in the last row in the Main menu.
Protocols are stored according to the plate type and you will find the protocol by first selecting the plate type, and then selecting it from the list.
What is the part number for re-ordering strip plates used for calibration?
You can use Nunc plates with the catalog number 473709.
I need to dispense a different volume into each well of the plate. How do I do that?
You can use the Multidrop Combi nL for that. First you need to select the volume for each well using FILLit Software. You can also copy/paste volumes into the plate layout.
The liquid is frothing during cell dispensing. How can that be minimized?
The cell culture media contains serum which can cause frothing. You can try lower dispensing speed, use lower mixing speed and use prime to empty to tubing to avoid excess foaming with empty function.
The tip of dispensing cassette was blocked during cell dispensing. What is recommended?
Use mild detergent or cleaning solution. You can let the detergent stand in the tubing 5-10 minutes to dissolve the clog. Then prime and empty by turns with the detergent and rinse well with several times with distilled water.
0 Items 
