Fluoroskan Ascent FL-Thermo Scientific

Fluoroskan Ascent FL Microplate Fluorometer and Luminometer

	Thermo Scientific* Fluoroskan Ascent FL Microplate Fluorometer and Luminometer is ideal for life science research applications.

Product Detail

	The Thermo Scientific Fluoroskan Ascent FL Microplate Reader is a compact and robust instrument with excellent optical performance for a variety of fluorometric and luminometric research applications, offering versatile plate formats, fast reading speeds, up to three dispensers and top/bottom reading of plates. Fast reading speed Reads a 96-well plate in just 15 seconds, which is essential for kinetic applications Great for a wide variety of applications, such as cytotoxicity, ion channel, FRET and BRET Combination of fluorometric GFP measurement and a lumometric luciferase measurement from the same well is fast due to the versatility of assay programming Onboard dispensers for exact follow-up of kinetic reactions Equipped with up to three reagent dispensers for rapid kinetic assays, such as Ca²⁺ flux Supports simultaneous dispensing and reading, enabling monitoring of kinetic measurements from the very start of the reaction Very low dead volume and the backflush capability allow conservation of expensive reagent High sensitivity for both top and bottom reading Fiberless direct illumination optics for both top and bottom reading High sensitivity, wide dynamic range and low cross talk ensure accurate and precise results for 1- to 384-well plates Specially designed for automation Reads up to 384-well plates for increased assay throughput Easily integrated with automated systems Includes: PC Software and filter pairs: Excitation: 355nm/Emission: 460nm, Excitation: 485nm/ Emission: 538nm Ideal for: Ca2+ flux assays; Cell proliferation; Cytotoxicity; Cell adhesion; DNA quantitation; Reporter gene assays; Hybridization assays; Quantitation of PCR products; FRET assays; BRET assays; Molecular beacon assays; Immunoassays; Enzyme activity; Bacterial quantitation; Phagocytosis; Oligonucleotide assays Warranty and Service Offering: 1 year

Fluoroskan Ascent FL Microplate Fluorometer and Luminometer

Product#	Price	Description

5210450	-	Fluoroskan Ascent FL 100-240 V	Select
5210460	-	Fluoroskan Ascent FL 100-240 V, including one dispenser	Select
5210290	-	Wide wavelength range option (PMT); emission wavelength range 360 nm - 800 nm	Select

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Specifications:

Dynamic Range :

Fluorometry: >6 decades
Luminometry: >9 decades over whole gain setting area

No of dispensers :

Up to 3

Excitation Filters :

Up to eight filters in the excitation filter wheel. 355 and 485nm filters included as standard. Other filters available upon request.

Light Source :

Quartz-halogen Lamp

Detector :

Photomultiplier Tube

User Interface :

Requires, but does not include, a personal computer

Shaking :

Orbital shaker

Measurement Speed :

15 seconds, 96-well plate

Excitation Wavelength Range :

Excitation: 320- 700nm; Emission: 360 - 670nm

Incubator :

From ambient +3°C to 45°C, at ambient 25°C

Sensitivity :

Fluorometry: 2 fmol fluorescein/well in a black 96-well plate
Luminometry: 40 amol ATP/well using flash reaction, white 384-plate

Emission Filters :

Up to six filters in the emission filter wheel. 460 and 538nm filters included as standard. Other filters available upon request.

Dispensing Speed :

25 seconds, 96-well plate, 5µL/well

Dispensing volume :

1 to 1000µL in 1µL increments

Weight :

Basic unit: 21kg (46 lbs.); three optional dispensers add 3.5kg

Computer Interface :

Serial RS-232C port

Plate Types :

1- to 384-well plates