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Varioskan Flash Multimode Reader
Thermo Scientific* Varioskan Flash spectral scanning multimode reader offers optimal performance for demanding research assays.
Product Detail
The Varioskan Flash provides flexibility for a variety of different applications with unlimited wavelength selection, up to three onboard dispensers, unparalleled optical performance and the advanced Thermo Scientific SkanIt* Software. The Varioskan Flash Multimode Reader has passed the DLReady* validation at Promega Corporation* and is certified as DLReady. Click here to view certification information The Varioskan Flash Multimode Reader has been granted the LanthaScreen* Certified designation by Invitrogen*. Click here to view certification information Varioskan Flash spectral scanning multimode reader includes fluorescence intensity, time-resolved fluorescence (TRF), photometric, and optional luminometric detection technologies. It is designed for optimization and analysis of various assays, such as binding assays, ADMETox, molecular biology assays, enzyme kinetic studies, ion-channel and cell signalling assays.Unlimited wavelength selection for spectral analysis and measurement at any single wavelength—the optimal measurement wavelengths can be identified and easily selected for any assay at any time Extremely easy measurement setup with automated internal functions ensuring maximal sensitivity and full dynamic range for every assay Onboard dispensers for exact follow-up of kinetic reactions—essential for flash luminescence assays, Ca2 + flux studies and other rapid kinetic applications Increased assay throughput—reads up to 1536-well plates and can easily be integrated with automated systems High performance incubator for temperature critical assays Easy assay setup, flexible data handling and convenient report formatting with SkanIt Software Ideal for: Apoptosis assays; Ca2 + flux assays; Cell proliferation; Cellular assays; Cytotoxicity and ADMETox; Direct DNA, RNA and protein quantitation; ELISA/FIA/TRF-ELISA assays; Enzyme kinetic studies; Europium assays; FRET assays; TR-FRET assays; BRET assays; GPCR assays; Ion channel assays; Kinase assays; Multilabel assays; Reporter gene assays; Signal transduction; Tryptophan and tyrosine UV fluorescenceWarranty and Service Offering: 1 year
Varioskan Flash Multimode Reader
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Specifications:
Incubator Temperature Range : From ambient +4°C to 45°C, at ambient 25°C
Precision (Photometry) : SD <0.001 Abs or CV <0.5%, whichever is greater, at 450nm (0 to 3 Abs)
No. of Dispensers : Up to 3
Sensitivity (Luminometry) : <7 amol ATP/well, flash ATP reaction, 384-well plate
Dynamic Range (Luminometry) : >7 decades
Spectral Scanning Speed : <2 s/well, 400 to 500nm, 1 flash, 2nm steps
Weight : 55 to 67kg (121-148 lbs.), depending on the configuration
Accuracy (Photometry) : ±2% or 0.003 Abs, whichever is greater, at 200 to 399nm (0 to 2 Abs) ±1% or 0.003 Abs, whichever is greater, at 400 to 1000nm (0 to 3 Abs)
Wavelength Range (Photometry) : 200 to 1000nm
Wavelength Range (Luminometry) : 360-670nm, spectral scanning 270-840nm
Plate Types : Fluorometry: 6- to 1536-well plates Luminometry: 6- to 1536-well plates Photometry: 6- to 384-well plates
Wavelength Selection : Fluorometry: Double excitation and double emission monochromators Luminometry: All wavelengths, filters and double monochromators Photometry: Double monochromators
Sensitivity (Fluorometry) : Fluorescence intensity, top reading: <0.4 fmol fluorescein/well, 384-well plate Fluorescence intensity, bottom reading: <4 fmol fluorescein/well, 384-well plate Time-resolved fluorescence, top reading: <120 amol Europium/well, 384-well plate
Dynamic Range (Fluorometry) : Fluorescence intensity, top reading: >6 decades Fluorescence intensity, bottom reading: >5.5 decades Time-resolved fluorescence, top reading: >6 decades
Linearity (Photometry) : 0 to 4 Abs, (96-well plate) at 450nm, ±2% 0 to 3 Abs (384-well plate) at 450nm, ±2%
Light Source : Xenon flash lamp
Measurement Types : Fluorescence intensity, time-resolved fluorescence, photometry and optional luminometry, all with spectral scanning
Shaking : Orbital with adjustable speed and diameter
Measurement Speed : 96-well plate in 15 seconds, 384-well plate in 45 seconds, and 1536-well plate in 135 seconds (minimum kinetic interval time from A1 back to A1)