|
|
|
|
 |
|
Life Science Mass Spectrometry
| Tip of the Month: Implementing automated Xreports in QuickQuan™ |
 |
Thermo Scientific QuickQuan software generates Excel summary files automatically that contain numeric results, but templates from Xreports can also be utilized to create more customized reports. After designing a report template, enable the sample or summary report option in the QuickQuan generic processing method template (.pmd). After QuickQuan completes the automated data processing, the Xreport will be created and stored in the time-date stamped data folder.
Learn more
|
| Proteomics: Quantitative Proteomic Workflow for Discovery of Early Rejection Kidney Transplant Peptide Biomarkers and Subsequent Development of SRM Assays in Urine |
|
Described in this application note is the development of a workflow utilizing off-line size fractionation coupled with on-line liquid chromatography and high resolution tandem MS on a Thermo Scientific LTQ Orbitrap XL hybrid mass spectrometer specifically geared toward the identification of differentially expressed proteins and endogenous peptides in urine. Selected protein or peptide biomarkers identified in the high-resolution discovery workflow were subsequently quantified in targeted selective reaction monitoring (SRM) assays on a Thermo Scientific TSQ Quantum Ultra triple quadrupole mass spectrometer.
Download Full Article [PDF 702 kB]
|
|
| Proteome Digest eNewsletter |
|
|
Proteome Digest eNewsletter offers timely, relevant information about new tools, workflows, and research results in proteomics. It provides links to free Web seminars, articles, application notes, interviews with experts, the latest in proteomics technology, and more...
Learn more and Subscribe
|
| Forensic Analysis: Identification of Lysergic Acid Diethylamide (LSD) in Candy by UHPLC/MS |
|
Lysergic acid diethylamide (LSD) is a controlled substance in forensic chemistry that is notorious for being difficult to identify. Most forensic laboratories confirm the presence of LSD by using gas chromatography with mass spectrometry (GC/MS). The disadvantages of GC/MS are its requirements for extensive sample preparation, including chemical derivatization of LSD to a more volatile form, and its impaired performance with analytes that are polar, thermally labile, or nonvolatile. An alternative method to positively identify LSD in complex food matrices is to use ultra high performance liquid chromatography with mass spectrometric detection (UHPLC/MS). UHPLC/MS offers a threefold benefit compared to GC/MS; simpler sample preparation, no derivatization, and less time wasted baking out or cleaning the instrument.
Download Full Article [PDF 223 kB]
|
In collaboration with Jason R. Stenzel, Washington State Patrol - Crime Laboratory Division, Cheney, WA, USA
|
|
Selective Testosterone Analysis in Human Serum by LC-FAIMS-MS/MS
|
|
|
Current high throughput clinical assays utilizing triple quadrupole mass spectrometry for the quantitation of testosterone can be further enhanced through the use of FAIMS (high-Field Asymmetric waveform Ion Mobility Spectrometry) coupled to a TSQ Quantum triple quadrupole mass spectrometer. This application note describes how a FAIMS-enabled TSQ Quantum™ improves the performance of a testosterone assay.
Download Full Article [284 kB]
|
| Metabolomics: Enhanced LC-MS/MS Selectivity for the Analysis of Human Urinary 8-isoprostaine, using FAIMS |
|
A series of isoprostanes is generated non-enzymatically in humans as peroxidation reaction products of arachidonic acid. The 8-isoprostane (8-epi PG F2α) is a prostaglandin formed by free radical oxidation and has been proposed as a marker of antioxidant deficiency and oxidative stress. Isoprostanes are present in very low concentrations with high background; therefore, a highly sensitive and selective assay is needed to quantify these compounds accurately. A newly developed LC-FAIMS-MS/MS method for 8-isoprostane in human urine extract described in this application note provides high selectivity with short chromatographic separation time. The chromatogram of 8-isoprostane using FAIMS shows significantly improved selectivity and higher sensitivity than the conventional technique.
Download Full Article [307 kB]
|
|
| Food Safety: Case Study - LC-MS/MS Detection of Melamine |
|
|
When the presence of melamine was discovered in animal food sources in early 2007, the National Center for Food Safety and Technology (NCFST) needed to quickly learn more about the effects of food processing on the chemical to learn how to accurately detect the presence of melamine in food. In July 2007 the NCFST began using the TSQ Quantum Ultra™ triple stage quadrupole system coupled with the Thermo Scientific Accela high-speed chromatography system to develop its LC-MS/MS method to monitor melamine and its hydrolytic products in processed foods. The TSQ Quantum triple stage quadrupole system is the only instrument that enables Highly Selective Reaction Monitoring (H-SRM) performance, which facilitates the quick and efficient analysis of complex samples such as animal tissue. The Thermo Scientific LC-MS/MS solution has yielded accuracy and precision values for this method that were well within the guidelines of the U.S. Food and Drug Administration (FDA) for analytical method development and validation.
Download Full Article [PDF 210 kB]
Press Release [PDF 144 kB]
|
| Columns: High Sensitivity, Flexible Silica Columns |
| Thermo Scientific columns are available in nanobore formats for nanospray LC/MS applications. At flow rates of nL/min versus mL/min, these columns offer higher sensitivity with greater signal-to-noise ratio than traditional capillary columns used with electrospray. Analytes can be detected, identified and quantified at femtomole levels and below. Full details of these columns can be found on the Product Specification Sheet.
Download Product Specification Sheet [398 kB]
Learn more
|
|
| Live Webinar: Simplify LC-MS Screening and Quantification using the Thermo Scientific Exactive MS (January 14th, 2009, 11 AM EST/5 PM CET) |
|
|
The new Exactive™ bench top LC-MS instrument has high resolution, accurate mass and fast scanning capabilities which make it ideally suited for both screening and quantification of compounds in complex samples. During this webinar, the presenters will describe the overall system performance and specifications together with a brief review of Orbitrap™ technology. Specific examples of how high resolution mass spectrometry simplifies quantification of hormones in meat products, pesticides in animal food matrix, and drug metabolites will be discussed.
Who should attend?
-
Analytical chemists using mass spectrometry in food, environmental, industrial, and pharmaceutical labs
-
HPLC, LC-MS, LC-MS/MS and LC-ToF users
Learn More and Register
|
| Trap Talk: Promotional Offer - Trade Up to the Power of Linear Ion Trap Technology |
|
Trade in your Thermo Scientific LCQ series ion trap for our industry-leading linear ion trap mass spectrometer technology. Don't miss out on this limited time promotional trade-in offer to improve the analytical capabilities of your laboratory with a new LTQ XL™ ion trap.
Why trade up?
- Experience superior sensitivity
- Extend performance further with an upgrade path to the LTQ Orbitrap XL™
- Generate data faster, with more confident data interpretation from new application-specific software
» Request More Information Now
|
|
|
|
|
|
|
|
|
|
|
