Tip of the Month: Reduce the Cost of Helium Per Sample

Proteomics: Efficient Enrichment and Identification of Cell Lysate Phosphopeptides Using an Automated, Magnetic Bead-Based System Coupled to High-Resolution LC-MS/MS and SRM 

Phosphoproteins are integral to global cellular signaling in disease and key to understanding biological regulation. Unfortunately, many phosphopeptides are present at very low levels in a typical cell lysate. An efficient, automated and robust enrichment strategy in a proteomics workflow can facilitate the identification and quantification of these lower-abundance species. Previous approaches have focused on enrichment with immobilized metal ion affinity chromatography (IMAC). However, enrichment and recovery of phosphopeptides using an IMAC system strongly depends on the type of metal ion and column material, and is often hampered by the non-selective enrichment of acidic peptides.

This application notes describes an integrated, automated, high-throughput workflow using high-resolution LC-MS/MS for the selective enrichment and identification of phosphopeptides from cell lysates.

» Download Complete Application Note [PDF 617 kB]

Neat calibration curve of synthetic phosphopeptide FLpSQDAPTVK

Proteomics: Online Training Course

Metabolism: Simultaneous Metabolite Identification and Quantitation of a Parent Drug Using Reverse Energy Ramp Scanning on a Triple Stage Quadrupole Mass Spectrometer

Recent advances in the field of mass spectrometry have enhanced and expanded the capabilities of triple stage quadrupole (TSQ) mass spectrometers. These innovations include using linear ion traps to increase the product ion sensitivity; using high-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) to increase the selectivity; and, more recently, using reverse energy ramp (RER) scanning to increase the product ion sensitivity. In addition, software developments, such as SRM-triggered data dependent scanning, have enabled the simultaneous acquisition of quantitative SRM data and qualitative product ion mass spectra on a TSQ instrument.

This application note describes the quantitation of loperamide, an anti-diarrheal agent, in mouse plasma and the simultaneous identification of its metabolites. Both data dependent scanning and reverse energy ramp scanning have been used to enhance the product ion sensitivity on a triple stage quadrupole mass spectrometer. Quantitation-Enhanced Data-Dependent MS/MS (QED-MS/MS) scanning delivers an information rich mass spectrum that can be used to confirm the existence of compounds while they are being quantified.

» Download Complete Application Note [PDF 507 kB] 

Structure of loperamide

Toxicology: Screening Drugs and Toxic Compounds with LC-MS/MS: An Alternative to LC-UV for Research Toxicology Labs

QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) is a dispersive Solid Phase Extraction (SPE) technique for extracting multi-residues of pesticides from fruits and vegetables. The advantages of this methodology are speed, ease of execution, minimal solvent requirement and cost to perform when compared with conventional solid phase extraction techniques. This application note demonstrates QuEChERS dispersive SPE as a simple, fast and quantitative sample preparation method for the GC/MS analysis of pesticides in grapes.

» Download Complete Application Note [PDF 868 kB]

18th IMSC 2009 (August 30 - September 4, Bremen, Germany)

Visit the Thermo Scientific booth at the 18th IMSC 2009 in Bremen, Germany to find out about new methods and instrumentation for resolving complexity in your mass spectrometry analyses.

Participation in all Thermo Scientific events is free of charge. However, capacity is limited, therefore please pre-register your interests.

• Thermo Scientific Users' Day
  Sunday, August 30, 2009, 10:30 - 15:30 h
• Lunchtime Workshops
  Monday, Tuesday, Wednesday, August 31, September 1 and 2, 2009
  12:15 - 13:45 h 
• Hospitality Suites
  Monday and Tuesday, August 31 and September 1, 2009
  18:15 - 21:00 h 
• Thermo Fisher Scientific Job Opportunities
  Thursday, September 3, 2009

» Learn more and Register

Upcoming Events 

Resolving Complexity

Visit the Thermo Scientific booths at these events for more information about products and workflows to meet your challenges. 

Europe

» 18th IMSC 2009, August 30 - September 4, 2009, Bremen, Germany [Mass Spectrometry]

» EUROANALYSIS 2009, September 6-10, 2009, Innsbruck, Austria [Food Safety & Environmental]

» Mycotoxin Forum, September 9-11, 2009, Tulln, Austria [Food Safety & Environmental]

» 46th Congress of the European Societies of Toxicology (EUROTOX), September 13-16, 2009, Dresden, Germany [Toxicology]

» Environmental Health Risk 2009, September 21-23, 2009, New Forest, UK [Environmental]

North America

» Applied Pharmaceutical Analysis (APA) BSAT, September 13-17, 2009, Boston, MA, USA [Bioanalysis]

» 123rd AOAC Annual Meeting & Exposition, September 13-16, 2009, Philadelphia, PA, USA [Food Safety & Environmental]

» Mass Spec 2009, September 15-18, 2009, Philadelphia, PA, USA [Mass Spectrometry]

» HUPO 8th Annual World Congress, September 26-30, 2009, Toronto, Canada [Proteomics]

Asia Pacific

» JAIMA, September 1-4, 2009, Tokyo, Japan

Trap Talk: Improved Sensitivity Through Enhanced Ion Transmission on the new LTQ Velos™ Ion Trap 

Efficient transfer of ions from the ion source to the mass analyzer is one of the most crucial aspects of atmospheric pressure ionization (API) mass spectrometry. Most ion losses occur during this transfer, and those losses directly affect a mass spectrometer’s limits of detection and quantification. The bulletin describes the novel stacked-ring ion guide (S-lens) used in the Thermo Scientific LTQ Velos to significantly improve transmission of ions from the ion source to the ion trap mass analyzer.

» Download Complete Product Support Bulletin [PDF 259 kB]