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Life Sciences Mass Spectrometry
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Tip of the Month: Want Fewer Peaks in Your Chromatogram?
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Watch the ion mobility tutorials created to show you how to optimize your high-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) systems for the best results. The first tutorial shows how to optimize a compound and the second demonstrates how to put Thermo Scientific FAIMS onto the MS. Ask your sales specialist for the DVDs.
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Proteomics: Protein Quantitation
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Improving Quantitation of TMT-Labeled Peptides Using Stepped Higher-Energy Collisional Dissociation
Isobaric stable-isotope tagging technologies such as Tandem Mass Tags (TMT®) and Isobaric Tags for Relative and
Absolute Quantification (iTRAQ) are frequently used to enhance relative quantification of proteins in cells, tissues, and body fluids. Higher-energy collisional dissociation (HCD) is an MS/MS fragmentation technique that operates over a wide m/z range, making it well-suited to identification and
quantitation of isobarically labeled peptides. This application note demonstrates how the use of HCD with stepped, as opposed to fixed, collision energy can improve both the number of peptides quantified and the reproducibility of quantitation.
» Download Application Note 483:
Improving Quantitation of TMT-Labeled Peptides Using Stepped HCD
[PDF 658 kB]
An Evaluation of Three Peptide Quantitation Strategies for Serum Samples Using Liquid Chromatography-Selected Reaction Monitoring
When measuring peptides with triple quadrupole based LC-SRM assays in complex biological matrices, there are several issues that need to be addressed. First, each sample has a unique set of interferences and ionization effects. With peptide quantitation, however, there is an additional layer of
complexity. Unlike small molecules, where a BSA solution is typically used as a serum substitute, there is no equivalent matrix substitute for a serum or plasma digest. As a result, peptide calibration curves with real serum digest backgrounds have endogenous peptides present in them. Methods and
post acquisition data analysis have to take the endogenous peptide that is present in the background matrix into account.
This poster shows the evaluation of three peptide quantitation strategies using liquid chromatography and selected reaction monitoring mass spectrometry.
» Download Poster:
An Evaluation of Three Peptide Quantitation Strategies for Serum Samples
[PDF 243 kB]
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Bioanalysis: Case Study - Decrease Method Time and Increase Specificity in Bioanalysis
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The Metabolism and Pharmacokinetics Department at sanofi-aventis in Alnwick, UK provides bioanalytical support for projects and studies generated by the company's scientists around the world. The team's advanced triple stage quadrupole mass spectrometry instruments are pushed to the limit by
complex matrices, such as plasma and urine. These samples often create interferences that can invalidate assay results. While accuracy and precision are top priority, the laboratory must also be ready to run thousands of samples each week to meet aggressive production deadlines.
This Case Study shows how the team at sanofi-aventis incorporated complementary solutions - the Thermo Scientific TLX
system and FAIMS - helping them meet the accuracy, precision and high throughput challenges.
» Register to Download the Complete Case
Study:
Decrease Method Time and Increase Specificity in Bioanalysis
[PDF 657 kB]
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Stuart McDougall (left) and Michael Blackburn pictured with their Thermo Scientific TSQ Vantage mass spectrometer equipped with selectivity-enhancing FAIMS
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Food Safety: Non-targeted Screening and Accurate Mass Confirmation of 510 Pesticides on the High Resolution Exactive Benchtop LC/MS Orbitrap Mass Spectrometer
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As agricultural trade grows and food safety concerns mount, stricter pesticide regulations are being enforced around the world. Increased pesticide testing and reductions in maximum permissible residue levels have driven demand for fast, sensitive and cost-effective analytical methods for
high-throughput screening of multi-class pesticides in food.
This application note shows the detection of 510 pesticides at low ppb levels was achieved within 12 minutes using the Thermo Scientific Exactive benchtop LC/MS system powered by Orbitrap technology. The Thermo Scientific Orbitrap technology enables accurate mass confirmation of all compounds, including isobaric pesticides. The Exactive™ LC/MS is ideally suited for routine,
comprehensive screening of targeted and non-targeted pesticides at or below the 0.01 mg/kg (10 ppb) default limit set by EU and Japanese legislation.
» Download the Complete Application Note 51878:
Non-targeted Screening and Accurate Mass Confirmation of 510 Pesticides
[PDF 2 MB]
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Chromatography: LC/MS Solvents and Columns
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Fisher Chemical Optima® LC/MS Solvents Improve Results
Choosing the right solvent for LCMS can improve the chromatographic or mass spectroscopic properties of the analyte as well as the overall detection limits of the instrument setting. Distributed
by the Fisher Chemical product line, Optima® LC/MS solvents are manufactured
using additional purification processes, quality control measures, and packaging innovations in order to meet the required purity level of advanced LCMS systems.
» Download
the Fisher Chemical LC/MS Whitepaper
[PDF 1 MB]
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Thermo Scientific Column Oven: Exact Temperature Control for LC Columns
Thermo Scientific Column Oven delivers efficient temperature control of HPLC columns, providing the highest levels of reproducibility and stability.
• Offers temperature control from ambient to 90°C
• Fits up to four columns in one compartment
• Compatible with CTC autosamplers and Thermo Scientific MS ion sources
The simple and fast assembly of Column Oven incorporates unique holding adapters, which allows the unit to fit easily into existing systems.
» Download the Thermo Scientific Column Oven Product
Specifications Sheet
[PDF 111 kB]
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New Additions to Thermo Scientific Hypersil GOLD Range of HPLC Columns
To complement the already extensive range, Hypersil™ GOLD columns are now also be available in five new phases:
• Hypersil GOLD C4
• Hypersil GOLD Amino
• Hypersil GOLD
• Hypersil GOLD SAX
• Hypersil GOLD Silica
With a total of 11 phases now available, the Hypersil GOLD family is now one of the most comprehensive ranges of columns on the market. All phases use the same highly pure silica backbone, delivering outstanding peak shape, whether an application calls for reversed phase, ion exchange, HILIC or
normal phase chromatography.
With all phases being available with 1.9 µm particle size the expanded range of Hypersil GOLD columns now offers chromatographers even more flexibility in choosing the correct column, whether they are using conventional or ultra-high pressure LC systems.
» View the interactive online version of the Thermo Scientific Chromatography Columns and Consumables Catalog (pages 350 to 369) for more information on the Hypersil GOLD range of HPLC Columns.
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EPA Analytical Methods: Gulf of Mexico Oil Spill Response - Environmental Solutions Using GC, GC-MS, AA, ICP and ICP-MS Systems
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With the recent off shore oil spill impacting the Gulf Coast region Thermo Fisher Scientific is prepared to provide the tools for water and sediment sample analysis. Thermo Fisher Scientific has the solutions to help you protect the region!
Thermo Scientific instrumentation, software and consumables offer complete solutions for the EPA Methods published in the EPA Quality Assurance Sampling Plan. The Thermo Scientific GC, GC-MS, AA, ICP and ICP-MS systems comply with the EPA analytical methods required. For your
convenience these solutions are outlined in this booklet.
» View the Interactive Thermo Scientific Gulf of Mexico Oil Spill Response Flipbook
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Trap Talk: Kinetic Energies of Ions Ejected Radially from a Linear Ion Trap at High Q
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Fundamental studies of ion trap operation have included measurements of ion kinetic energies (KE) upon ejection either at the boundary of stability or through a resonance ejection process. Most of the previous measurements were performed at low q<0.4 which allowed comparison with theoretical
values from the pseudo-potential model [1,2.] Ejection at high q>0.4 on a 3D ion trap at the mass stability boundary was investigated in showing significant increase in average KE compared to low q ejection. The goal of this work is to investigate experimentally the effects of various ion trap
geometries and ejection conditions on the KE distribution of ejected ions at high q. In addition, all of these studies are performed on 2D linear ion traps (LIT). Phase effects of the phase-locking of the resonance excitation to the main RF on the ion KE are also demonstrated.
» Download Poster:
Kinetic Energies of Ions Ejected Radially from a Linear Ion Trap at High Q
[PDF 243 kB]
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